Advances in Miniature Multiphoton Imaging

Miniaturized Multiphoton Microscopy
—Advancing Neuroscience In Vivo Imaging

Miniaturized multiphoton microscopy represents a transformative advancement in in vivo imaging technology. By integrating the high-resolution, deep-tissue imaging capabilities of multiphoton microscopy with compact, wearable designs, this innovative approach enables real-time, subcellular-level imaging in freely moving animals.

This fusion of performance and portability opens new frontiers in neuroscience, physiology, and developmental biology by allowing researchers to study dynamic biological processes in naturalistic settings with minimal disruption.

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Where Innovation Meets Insight
— The TRANSVISTA Advantage

Features
• Deep tissue penetration

• Intrinsic optical section

• High signal to noise ratio

• Low photobleaching

Exceptional optical path design
• Multi-color imaging

• Multi-probe imaging

• Optogenetic module

Excellent mechanical design
• Easy plug in and plug out

• Long term imaging

Technology, Transcended

Sub-cellular Resolution with Cranial Window and GRIN Lens

Technology, Transcended

Continuous Imaging

Technology, Transcended

Long-Term Imaging

Repeated imaging of the same neuronal
population over multiple months

X,Y axis offset within 10 μm

Low photobleaching enables continuous imaging

Continuous imaging over 24 hours

Hour 0

Hour 19

Hour 22

Frame rate: 5Hz

50 μm

Superior resolution with both cranial window and GRIN lens

Monitor neuronal activity/ structural changes in the entire brain using either cranial window (up to 800 μm) or GRIN lens.

Imaging through cranial window

with LF probe

Imaging via GRIN lens in CA1

with HR probe

Imaging through cranial window

with LF probe

Imaging via GRIN lens in CA1

with HR probe

Technology, Transcended

Volumetric imaging

The Electrical Tunable Lens (ETL) enables 3D-imaging

Intrinsic sectioning property enables recording of neuronal
dynamics/structures in adjacent layers

0 μm

-150 μm

(The ETL adjustable focal range depends on the headpiece. For detailed specifications, please refer to the ' Two-Photon Headpieces ' section)

The ETL enables simultaneous recording of two different layers

Dendritic layer at -50 μm

Soma layer at -140 μm

Publications

Nature Methods

A versatile miniature two-photon microscope enabling multicolor deep-brain imaging.

Advanced multi-color excitation, deep brain imaging, and cross-scale neural observation — expanding new possibilities for neuroscience.

21 August 2025

Nature Methods

Miniature three-photon microscopy maximized for scattered fluorescence collection

Incorporated three-photon imaging, enabling non-interventional deep brain observation in the hippocampus.

23 February 2023

Nature Methods

Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging

Expanded the imaging field-of-view by 7.8x and introduced 3D imaging capabilities.

06 January 2021

Nature Methods

Fast high-resolution miniature two-photon microscopy for brain imaging in freely behaving mice

Developed a 2.2g microscope, achieving the first clear and stable functional imaging of neural synapses in freely moving mice.

29 May 2017

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