Tailored according to the body shape of mice, it can stably fix awake mice, alleviate the interference of breathing or body shaking on experimental results during in vivo experiments, and relieve the difficulty of using anesthesia reagents to conduct mouse experiments. By combining techniques such as two-photon or confocal microscopy, photogenetics, and patch clamp, it can be used for in vivo experimental studies such as imaging or electrophysiology in conscious mice.