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Unveiling the Unseen

The Miniature Three-Photon Microscope SUPERNOVA-3000 is equipped with a miniature three-photon headpiece featuring a novel optical configuration design. This effectively increases the efficiency of scattered fluorescence collection, reaches the limit of benchtop three-photon imaging depth, and offers fast scanning speed with clear imaging.
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Image for reference only; actual product may vary slightly
Features

Long-wavelength femtosecond laser enables calcium imaging at a depth up to 1.2 mm

See through the entire cerebral cortex and corpus callosum, reaching the hippocampus of mice brain

Go Deeper

2.5g miniature headpiece, easily mounted on small animals

ETL-enabled three-dimensional imaging

Unique novel optical design, integrated with cutting-edge technologies such as flexible light-shielding optical fibers, hollow-core optical fbers and MEMS

More freedom

Deep brain imaging only requires cranial window surgery, avoiding damage to brain tissues caused by implanted Grin lens.

Longer wavelength of excitation light, lower phototoxicity

Configuration enhances collection of scattered fluorescence, allowing for deep brain imaging using a safe level of laser power

Less Damage
Miniature Headpieces FHIRM-D
Notes
Lateral Resolution @1300 nm
1.2 μm
Notes
FOV*
360*360 μm
Notes
Working Distance
1.6 mm
Notes
Frame Rate*
15 Hz@128×128
Notes
Optical specifications

* The laser repetition rate should be ≥400 kHz

Design and specifications are subject to change without prior notice.

Supple fiber bundle
Laser collimator

Miniature objective

(TVS-D series)

MEMS scanning

mirror

Applications
3D Reconstruction of Mouse Brain Tissues
3D reconstruction imaging of blood vessels from the mouse cortex to the hippocampus region

Green: FlTC-Dextran dye

lmaging depth:0~1428 μm

Excitation wavelength: 1320 nm

Head-fixed imaging of awake mice

3D reconstruction imaging of neurons from the mouse posterior parietal cortex to the hippocampal CA1 subregion

Green: AAV-hSyn-GCaMP6s

Magenta: Third harmonic signal

Imaging depth: 0~1236 um

Excitation wavelength: 1320 nm

Head-fixed imaging of awake mice

3D reconstruction imaging of neurons from the mouse posterior parietal cortex to the hippocampal CA1 subregion

Green: Thy1-YFPH

lmaging depth: 0~1038 μm

Excitation wavelength:1320 nm

Head-fixed imaging of awake mice

3D reconstruction imaging of mouse cerebral cortex to Postsubiculum

Green: AAV-hSyn-GCaMP6f

lmaging depth: 0~1000 μm

Excitation wavelength:1320 nm

Head-fixed imaging of awake mice

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